Methods of producing bioconjugates of E. coli O-antigen polysaccharides, compositions thereof, and methods of use thereof
US12233121B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 6, 2022 |
| Grant date | Feb 25, 2025 |
| Priority date | — |
| Expiry date | Oct 12, 2042 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate to be produced. The oligosaccharyl transferase enzymes can be PglB oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein to vaccinate a subject against extra-intestinal pathogenic E. coli. (ExPEC).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.