Efficient deposition of nano-sized particles onto cells at an air liquid interface
US12234440B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 25, 2020 |
| Grant date | Feb 25, 2025 |
| Priority date | — |
| Expiry date | Sep 3, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2513/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus and method for the delivery and deposition of particles air-liquid-interface (ALI) cell cultures includes a sample inlet coupled to a growth tube having interior walls that are wet. The growth tube is configured to operate at a first temperature along a first length of the tube and a second temperature along a second length positioned between the first length and a growth tube outlet. The apparatus also includes a nozzle plate having a plurality of nozzles. The exposure chamber adapted to hold cell cultures at an air-liquid interface positioned underneath the plurality of nozzles and a temperature regulator adapted to control a temperature of the exposure chamber. The apparatus also includes a controller including instructions operable to cause the controller to maintain a relative humidity within the exposure chamber by controlling at least the second temperature of the growth tube and the temperature of the exposure chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.