Cell expansion

US12234441B2 · kind B2 · utility

Assignee

• Terumo BCT, Inc. · US

Inventors

• Boah VANG · Northglenn, US
• Brian J. Nankervis · Golden, US
• Mark-Eric Jones · Bussigny, CH

Key dates

• Filing date: Nov 2, 2020
• Grant date: Feb 25, 2025
• Expiry date: Dec 28, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/2302
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.