Aspergillus microorganism carrying disruptions of multiple genes and a method of producing thereof
US12234466B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 4, 2019 |
| Grant date | Feb 25, 2025 |
| Priority date | — |
| Expiry date | May 17, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The objective of the present invention is to provide a transformed Aspergillus microorganism lacking at least two types of selection marker genes available for marker recycling method and a composition therefor. The objective can be achieved by a transformed Aspergillus microorganism lacking at least two types of selection marker genes available for marker recycling method on its chromosomes, or a composition for transforming an Aspergillus microorganism containing at least two types of nucleic acid fragments containing a loop-out region and a selection marker gene available for marker recycling method between homologous recombination regions, wherein the selection marker genes contain a tryptophan biosynthesis gene and a gene different from tryptophan biosynthesis gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.