Tiered ligation oligos
US12241059B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Jul 15, 2021 |
| Grant date | Mar 4, 2025 |
| Priority date | — |
| Expiry date | Sep 18, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure provides methods for creating long oligonucleotide reagents that include barcodes and other element for sequencing library preparation, where the oligonucleotides are created by multiple tiers of ligation of shorter oligos. The disclosed methods work to extend short oligos that are attached to particles, thereby allowing one to create particles that carry large number of long sample preparation oligonucleotides without being required to synthesize those full-length molecules with a polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.