3D printed micro-millifluidic bioreactors for long-term retinal organoid maintenance

US12247220B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• William C. Tang · Irvine, US
• Andrew W. Browne · San Joaquin Hills, US
• Magdalene J. Seiler · Louisville, US
• Yuntian Xue · Irvine, US

Key dates

• Filing date: Sep 6, 2022
• Grant date: Mar 11, 2025
• Expiry date: Sep 6, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/90
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A bioreactor device includes a solid substrate having a first face and a second face. The solid substrate at least partially defines a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet. A first sheet disposed over the first face and a second sheet disposed over the second face. Characteristically, the combination of the solid substrate, the first sheet and the second sheet define the perfusion channel and each chamber of the plurality of chambers. The plurality of chambers are arranged in rows of chambers in which adjacent chambers are positioned at opposite side of the perfusion channel. The perfusion channel extends from the fluidic inlet and the fluidic outlet having a serpentine path along each row of chambers with each chamber being in fluid communication with the perfusion channel.