Compositions and methods for improving sample identification in indexed nucleic acid libraries
US12247254B2 · kind B2 · utility
0Cited by
21References
17Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Feb 22, 2021 |
| Grant date | Mar 11, 2025 |
| Priority date | — |
| Expiry date | Jun 19, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B80/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.