Method of manufacturing agar or agarose beads

US12251681B2 · kind B2 · utility

Assignee

• Cytiva BioProcess R&D AB · SE

Inventors

• Susanna Klara Margareta Lindberg · Uppsala, SE
• Jonas Gustafsson · Uppsala, SE
• Linn Carlsson · Uppsala, SE
• Anders Hagvall · Uppsala, SE
• Erik Ringberg · Taylors, US
• David Jansson · Uppsala, SE

Key dates

• Filing date: Apr 28, 2020
• Grant date: Mar 18, 2025
• Expiry date: Apr 6, 2042

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/52
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and e) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.