Fluidic device, fluidic system, and method for developing three-dimensional cellular constructions

US12252677B2 · kind B2 · utility

Assignee

• Technische Universität Darmstadt · DE

Inventors

• Ulrike Nuber · Mühltal, DE
• Heinz Koeppl · Matrei in Osttirol, AT
• Steffen Hardt · Mainz, DE
• Katrin Töpfer · Darmstadt, DE

Key dates

• Filing date: Oct 28, 2019
• Grant date: Mar 18, 2025
• Expiry date: Jul 25, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M35/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A fluidic device, a fluidic system and a method for developing a cellular starting material into a three-dimensional cellular structure. The fluidic device includes a base body which includes a chamber in which a matrix is received, into which the cellular starting material to be developed can be introduced, and at least two fluid reservoirs. Each fluid reservoir includes a fluid inlet, a fluid outlet and a separating device which is partially permeable to a fluid medium and which separates the associated fluid reservoir from the chamber and forms a common plane interface with the chamber, via which the fluid medium can diffuse into the matrix. When using suitable fluid media, the fluidic device is adapted to form at least one concentration gradient, at least two mutually orthogonal concentration gradients and/or at least two mutually antiparallel concentration gradients in the matrix, each of which are essentially homogeneous or deliberately inhomogeneous in the z-direction over at least a section of the extension of the matrix.