Chimeric and other variant β-glucuronidase enzymes with enhanced properties
US12252719B2 · kind B2 · utility
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17References
26Claims
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Key dates
| Filing date | Jul 14, 2022 |
| Grant date | Mar 18, 2025 |
| Priority date | — |
| Expiry date | Jul 14, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01031
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Chimeric and other variant β-glucuronidase enzymes with enhanced properties as compared to unmodified enzyme are provided. The enzymes of the invention advantageously exhibit enhanced enzymatic activity, enhanced substrate range, enhanced pH range, enhanced temperature range and/or enhanced enzyme stability. Methods of using the variant enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.