Ketoreductase polypeptides and polynucleotides
US12252723B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 14, 2023 |
| Grant date | Mar 18, 2025 |
| Priority date | — |
| Expiry date | Jul 14, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y120/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.