Process for simultaneous production of citric acid and cellulolytic enzymes

US12252726B2 · kind B2 · utility

Assignee

• Indian Oil Corporation Limited · IN

Inventors

• Mukund Adsul · Faridabad, IN
• Simranjeet Kaur Sandhu · Faridabad, IN
• Reeta Rani Singhania · Faridabad, IN
• Anshu Shankar MATHUR · Faridabad, IN
• Ravi Prakash GUPTA · Sindhar, IN
• Deepak Kumar Tuli · Faridabad, IN
• Suresh Kumar Puri · Faridabad, IN
• Sankara Sri Venkata RAMAKUMAR · Faridabad, IN

Key dates

• Filing date: Jul 31, 2021
• Grant date: Mar 18, 2025
• Expiry date: Oct 15, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture of Penicillium funiculosum MRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.