Methods and compositions for the selective lysis of blood cells and separation of microbial cells
US12253513B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2019 |
| Grant date | Mar 18, 2025 |
| Priority date | — |
| Expiry date | Sep 23, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00495
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.