Methods for dynamic evolution and monitoring of characteristics in living cells using a microfluidic-enabled multi-well cell culture devices and systems
US12258551B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 2019 |
| Grant date | Mar 25, 2025 |
| Priority date | — |
| Expiry date | Dec 15, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6439
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.