Method and composition for quantifying protein using tagged standards
US12259356B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 7, 2021 |
| Grant date | Mar 25, 2025 |
| Priority date | — |
| Expiry date | Oct 9, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2496/15
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and reference compositions for quantifying protein using tagged standards. In an exemplary method, a reference composition and a protein may be electrophoresed in respective lanes of a gel. The reference composition may include quantitation standards of different size and each including a tag present at a different concentration. The quantitation standards and the protein may be transferred from the gel to a solid support to create a blot. Luminescence may be detected from the blot to obtain respective luminescence values separately representing an abundance of the tag of each quantitation standard and an abundance of the protein. A quantity of the protein may be determined using the respective luminescence values.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.