Protein sequencing via coupling of polymerizable molecules

US12259394B2 · kind B2 · utility

Assignee

• Glyphic Biotechnologies, Inc. · US

Inventors

• Asmamaw Wassie · Boston, US
• Daniel Masao Estandian · Cambridge, US
• Andrew J. Price · San Mateo, US
• Boyang HUA · Oakland, US
• Joshua Y. Yang · San Francisco, US
• David Dodd · Los Gatos, US
• Gerardo Fabian Delgado · Oakland, US
• Elaine Jean Su · Martinez, US

Key dates

• Filing date: Apr 4, 2024
• Grant date: Mar 25, 2025
• Expiry date: Apr 4, 2044

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/68
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers having an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.