Target enrichment by unidirectional dual probe primer extension

US12264315B2 · kind B2 · utility

Assignees

• Roche Sequencing Solutions, Inc. · US
• Kapa Biosystems, Inc. · US

Inventors

• Daniel Burgess · Black Earth, US
• Brian Christopher Godwin · Livermore, US
• Alexander Lovejoy · Newark, US
• Bronwen Miller · Cape Town, ZA
• Jo-Anne Elizabeth Penkler · Cape Town, ZA
• Joseph Platzer · Oakland, US
• Martin Ranik · Cape Town, ZA

Key dates

• Filing date: Aug 15, 2019
• Grant date: Apr 1, 2025
• Expiry date: Aug 15, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6876
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.