Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing
US12264364B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Dec 20, 2022 |
| Grant date | Apr 1, 2025 |
| Priority date | — |
| Expiry date | Feb 13, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are methods of preparing dual-indexed nucleic acid libraries for methylation profiling using bisulfite conversion sequencing. In various embodiments, the methods use a two-step indexing process to tag bisulfite-treated DNA with unique molecular identifiers (UMIs).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.