Method for determining ciprofloxacin in meat product based on enzyme-linked upconversion fluorescence and potassium titanium oxalate system
US12265032B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 3, 2024 |
| Grant date | Apr 1, 2025 |
| Priority date | — |
| Expiry date | Jul 3, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining ciprofloxacin in a meat product based on an enzyme-linked upconversion fluorescence and potassium titanium oxalate system is provided. An enzyme-linked upconversion nanoparticle solution is mixed with an aptamer-bound magnetized polydopamine solution to obtain a fluorescence-ultraviolet dual-signal detection system. A fluorescence standard curve and a colorimetric standard curve for the ciprofloxacin content determination are created through the fluorescence-ultraviolet dual-signal detection system in combination with potassium titanium oxalate and hydrogen peroxide. A fluorescence intensity signal characteristic value and an ultraviolet absorbance signal characteristic value of a meat sample solution are determined. A ciprofloxacin content in the meat sample is calculated according to the fluorescence standard curve and the colorimetric standard curve.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.