Manufacturing method for substrate on which nerve cells are arranged
US12270048B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 2, 2021 |
| Grant date | Apr 8, 2025 |
| Priority date | — |
| Expiry date | Mar 2, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2537/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells.A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrate, a plurality of liquid droplets containing nerve cells by an inkjet method to form one or a plurality of liquid pools, the substrate having a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and a step of incubating the liquid pool until the nerve cells sediment and temporarily adhere onto the substrate to form a cell aggregate. The diameter per one liquid pool is 500 μm or less, and the density of nerve cells per one liquid pool is 105 cells/cm2 or more.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.