Engineered polymerases
US12270056B2 · kind B2 · utility
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32Claims
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Key dates
| Filing date | Mar 25, 2022 |
| Grant date | Apr 8, 2025 |
| Priority date | — |
| Expiry date | Mar 25, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.
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