Feruloyl esterase and application thereof
US12270058B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 2021 |
| Grant date | Apr 8, 2025 |
| Priority date | — |
| Expiry date | Sep 24, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/125
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure discloses a feruloyl esterase and application thereof, and belongs to the technical field of microorganisms. The disclosure provides a feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2 and specific enzyme activity as high as 519 U/g. The disclosure provides a recombinant Bacillus subtilis which uses Bacillus subtilis as a host, expresses a gene encoding the feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2, and can be used for producing the feruloyl esterase with a high yield. The process specifically includes inoculating a fermentation culture medium with the recombinant Bacillus subtilis for fermentation for 14 hours, and the enzyme activity of the feruloyl esterase in a cell disruption supernatant can be as high as 82.53 U/mL.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.