Tagged nucleoside compounds useful for nanopore detection

US12270075B2 · kind B2 · utility

Assignee

• Roche Sequencing Solutions, Inc. · US

Inventors

• Frank Bergmann · Iffeldorf, DE
• Peter Crisalli · Mountain View, US
• Sara Kelley · Pleasant Hill, US
• Omid Khakshoor · Lathrop, US
• Hannes Kuchelmeister · Munich, DE
• Meng Taing · San Mateo, US

Key dates

• Filing date: Aug 25, 2020
• Grant date: Apr 8, 2025
• Expiry date: Nov 11, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing.