In vitro method of reprogramming fibroblast cells into induced pluripotent stem cells by transcription factors mRNAs co-transfected with SOCS1 mRNA wherein the mRNAs have a poly-A tail of at least 2000 adenines

US12275950B2 · kind B2 · utility

Assignee

• MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

• Marion Jurk · Dormagen, DE
• Stefan Wild · Hamburg, DE
• Andreas Bosio · Cologne, DE

Key dates

• Filing date: May 2, 2017
• Grant date: Apr 15, 2025
• Expiry date: Oct 27, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N5/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides the use of a nucleic acid encoding SOCS1 for enhancing the efficacy of introducing at least one nucleic acid of interest into a cell; a method of repeated transfection of a cell with at least one nucleic acid of interest comprising the steps of adding a) nucleic acid encoding SOCS1, and simultaneously or subsequently b) at least one nucleic acid of interest encoding at least one polypeptide of interest, wherein at least step b) is repeated at least once; and a method of electroporation of a cell with at least one nucleic acid of interest comprising the steps of adding to the cell a) a nucleic acid encoding SOCS1, and simultaneously or subsequently b) said at least one nucleic of interest. The at least one nucleic acid of interest and the nucleic acid encoding SOCS1 may be mRNAs, wherein each of said mRNAs has a poly(A) tail at its 3′ end comprising at least 200 adenines.