Compositions and methods for rapid and reversible biomolecular labeling
US12276658B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 22, 2013 |
| Grant date | Apr 15, 2025 |
| Priority date | — |
| Expiry date | Jul 19, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/7155
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.