Method of generating an inclusion list for targeted mass spectrometric analysis
US12276666B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2018 |
| Grant date | Apr 15, 2025 |
| Priority date | — |
| Expiry date | May 13, 2043 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/0027
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of generating an inclusion list for targeted mass spectrometric analysis is disclosed. Experimentally-acquired data for a plurality of isobarically-labeled peptides derived by proteolytic digestion of a corresponding protein. The data includes, for each of the isobarically-labeled peptides, a mass-to-charge (m/z) ratio, a charge state, and a chromatographic retention time (RT). The method includes determining a hydrophobicity index (HI) of an unlabeled peptide corresponding to the isobarically-labeled peptide. If the determined HI is less than a threshold value, a substitute unlabeled peptide is selected in accordance with predetermined criteria and predicted properties for the substitute peptide are determined and stored on an inclusion list. If the determined HI for the unlabeled peptide is at least as great as the threshold value, predicted properties for the unlabeled peptide are determined and stored on an inclusion list. The substitute unlabeled peptide may be selected from an available peptide library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.