Assembly of long nucleic acids by ligation using indexed splint oligos
US12281302B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Aug 2, 2022 |
| Grant date | Apr 22, 2025 |
| Priority date | — |
| Expiry date | Aug 2, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1093
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to a method of assembling long nucleic acids by enzymatically ligating oligonucleotide molecules hybridized to an indexed splint oligonucleotide molecules. Also disclosed are oligonucleotide structures comprising an indexed splint oligonucleotide useful in performing the disclosed method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.