Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
US12281316B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 30, 2023 |
| Grant date | Apr 22, 2025 |
| Priority date | — |
| Expiry date | Nov 30, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y401/01023
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.