GDSL lipase, genetically-engineered bacteria and application thereof
US12281337B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 27, 2023 |
| Grant date | Apr 22, 2025 |
| Priority date | — |
| Expiry date | Jun 27, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/19
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a GDSL lipase, genetically-engineered bacteria and an application thereof. The GDSL lipase is derived from Streptomyces diastaticus CS1801 and its amino acid sequence is as shown in SEQ ID NO.2. After construction of a genetically-engineered bacterium strain, a GDSL lipase is generated through fermentation. Through this enzyme, vitamin A and palmitic acid are converted to produce vitamin A palmitate. The content of the vitamin A palmitate obtained from the conversion is 16.35 mg/L at most. The conversion efficiency is 81.75% at most. This lipase provides a new path to synthesize vitamin A palmitate by the enzymatic method and has an important application prospect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.