Biotechnological production of diols

US12281343B2 · kind B2 · utility

Assignee

• SYMRISE AG · DE

Inventors

• Dirk Fabritius · Mainz, DE
• Stefan Lambrecht · Holzminden, DE
• Johannes Panten · Ankum, DE
• Marcus Eh · Holzminden, DE

Key dates

• Filing date: Jul 8, 2019
• Grant date: Apr 22, 2025
• Expiry date: Jan 21, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y101/0105
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for the stereo selective production of a trans-diol or a cis-diol or a hydroxyketone comprising the step(s) (i) conversion of a trans-diol or a cis-diol to a hydroxyketone and/or (ii) conversion of a hydroxyketoneto a cis-diol or a trans-diol, catalyzed by an enzyme, which is encoded by a nucleic acid sequence of SEQ ID NO: or wherein the enzyme comprises an amino acid sequence of SEQ ID NO: 2. The present invention also relates to the use of an enzyme encoded by a nucleic acid sequence of SEQ ID NO: 1 or wherein the enzyme comprises an amino acid sequence of SEQ ID NO: 2 for the conversion of a trans-diol to a cis-diol or for the conversion of a trans-diol or a cis-diol to a hydroxy ketone and/or the conversion of a hydroxyketone to a trans-diol or a cis-diol.