RNA and DNA analysis using engineered surfaces
US12281360B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 28, 2023 |
| Grant date | Apr 22, 2025 |
| Priority date | — |
| Expiry date | Jun 28, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5308
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and/or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA/RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for identification and/or localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA/RNA modifications in parallel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.