Methods for microscopy with ultraviolet surface excitation (MUSE) imaging
US12281985B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 2020 |
| Grant date | Apr 22, 2025 |
| Priority date | — |
| Expiry date | Aug 21, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0621
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described herein are methods for imaging a fluorescent bioassay (including a substrate, such as dispersed cellular sample, exposed to one or more exogenous fluorophore and/or fluorescent probe that accumulate in a structure of interest). The bioassay can be excited with Type-C ultraviolet (UVC) light produced by one or more light emitting diode (LED). The UVC can have a center wavelength that causes emission by the fluorescent bioassay. A digital optical device can collect a signal emitted from the fluorescent bioassay in response to the excitation. The methods relate in particular to Microscopy with Ultraviolet Surface Excitation (MUSE) imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.