Method and instrumentation for spatial multiomics using SUMI-technology

US12286665B2 · kind B2 · utility

Assignee

• MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

• Thomas Rothmann · Langenfeld, DE
• Robert Pinard · New Haven, US
• Hansueli Meyer · Uster, CH
• Andreas Bosio · Cologne, DE
• Heinrich Spiecker · Bielefeld, DE
• Seiyu Hosono · Mamaroneck, US
• Ryan Hindman · Moitzfeld, DE
• Emily Neil · Moitzfeld, DE
• Chris Nehme · Moitzfeld, DE

Key dates

• Filing date: Jan 31, 2022
• Grant date: Apr 29, 2025
• Expiry date: Aug 17, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.