Method of producing a sialylated N-glycosylated recombinant protein in periplasm of a recombinant Escherichia coli
US12291732B2 · kind B2 · utility
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Key dates
| Filing date | Jun 3, 2021 |
| Grant date | May 6, 2025 |
| Priority date | — |
| Expiry date | Dec 22, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/19
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein is a method for producing a sialylated N-glycosylated recombinant protein in periplasm of a recombinant Escherichia coli. The sialylated N-glycosylated recombinant protein is produced in the periplasm of a recombinant Escherichia coli strain W3110ΔnanKETA::Kan, and a sialylated oligosaccharide chain is Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,3-GlcNAc.
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