Patent · US Active

Cloning and expression system for T-cell receptors

US12297427B2 · kind B2 · utility

0Cited by

0References

11Claims

0Family size

Assignee

St. Jude Children's Research Hospital, Inc. · US

Inventors

Paul Thomas · Memphis, US
Xizhi Guo · Memphis, US
Pradyot Dash · Memphis, US

Key dates

Filing date	Dec 2, 2016
Grant date	May 13, 2025
Priority date	—
Expiry date	May 23, 2042

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC40B50/06
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides a method for rapid cloning of T-cell receptors (TCRs) (e.g., paired αβ and γδ TCR chains) and B-cell receptors (BCRs) (e.g. paired IgH or IgK or Igλ) from single cells by CDR3 substitution using single cell PCR products and Gibson Assembly techniques and a pre-generated TCR (or BCR) library in an expression vector.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.