Methods and probes for performing PCR with melt analysis for increased multiplexing
US12305223B2 · kind B2 · utility
0Cited by
2References
13Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 25, 2022 |
| Grant date | May 20, 2025 |
| Priority date | — |
| Expiry date | Dec 1, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2527/143
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.