Fluorescence microscope and method for imaging a sample
US12306103B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 16, 2020 |
| Grant date | May 20, 2025 |
| Priority date | — |
| Expiry date | Oct 25, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/0084
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence microscope includes excitation and de-excitation light sources designed to generate excitation and de-excitation light distributions, which excite and de-excite fluorophores present in a sample, respectively. An illumination unit is designed to combine the light distributions such that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another in an illumination target point. A detector is designed to detect the fluorescence photons as a function of their arrival times. The processor is designed to evaluate the detected fluorescence photons with respect to their arrival times and, based thereon, to control a delay which a light pulse or a light modulation of the de-excitation light distribution has at a position of the illumination target point in relation to a light pulse or a light modulation of the excitation light distribution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.