Method and device for identifying specific region in microorganism target fragment and use thereof
US12308093B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 14, 2020 |
| Grant date | May 20, 2025 |
| Priority date | — |
| Expiry date | May 14, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B35/20
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method for identifying presence of a microorganism in a sample has two processes. The first process comprises: S100, respectively comparing a microorganism target fragment with genome sequences of one or more comparison strain one-to-one, and removing fragments of which the similarity exceeds a preset value, to obtain a plurality of residual fragments as first-round cut fragments T1-Tn; S200, respectively comparing the first-round cut fragments T1-Tn with the remaining comparison strains, and removing fragments of which the similarity exceeds a preset value, to obtain a collection of residual cut fragments as a candidate specific region of the microorganism target fragment; and S300, verifying and obtaining a specific region. The second process comprises performing a PCR amplification of the specific region to detect the microorganism. The method is high in accuracy and sensitivity, which can identify the subspecies level and a dual-verification module is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.