Modified terminal deoxynucleotidyl transferase (TdT) enzymes
US12312611B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Feb 4, 2020 |
| Grant date | May 27, 2025 |
| Priority date | — |
| Expiry date | Feb 3, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07031
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Polμ, Polβ, Polλ, and Polθ of any species or the homologous amino acid sequence of X family polymerases of any species and their use in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of new terminal deoxynucleotidyl transferases and 3′-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.