Patent · US Active

Acid phosphatase mutants and methods of using the same

US12312612B2 · kind B2 · utility

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1References

1Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Liming Liu · Beijing, CN
Xin Xu · Dongying, CN
Wei Song · Houston, US
Xiulai Chen · Wuxi, CN
Jia Liu · Wuxi, CN
Cong Gao · Jingwei, CN
Jing Wu · New York, US
Liang Guo · Chicago, US

Key dates

Filing date	Mar 15, 2022
Grant date	May 27, 2025
Priority date	—
Expiry date	Sep 23, 2043

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y301/03002
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed is an acid phosphatase mutant obtained from Pseudomonas aeruginosa having the amino acid sequence of SEQ ID NO:3 and method of using the mutant in the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, the problems of a high substrate cost, environmental pollution and the like are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.