Engineering strain producing cordycepin and its derivative 3′-deoxyinosine, its preparation method and application
US12312625B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 2024 |
| Grant date | May 27, 2025 |
| Priority date | — |
| Expiry date | Apr 4, 2044 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of genetic engineering and process engineering, and discloses an engineering strain producing cordycepin and its derivative 3′-deoxyinosine, its preparation method and application. The method includes: codon optimizing genes Cm1 and Cm2; gene amplification and fragment recovering; methanol-induced promoter and terminator fragments; and inserting the genes into an expression vector and then transferring to Pichia pastoris for screening to obtain the engineering strain of Pichia pastoris of the present invention. The engineering strain can be applied to production of cordycepin and its derivative. The engineering strain prepared by the preparation method of the present invention has great advantages in producing cordycepin and its derivative. Due to the use of unique strains of Cordyceps militaris which have unique Cm1 and Cm2 genes, the efficiency of cordycepin production is greatly improved, and subsequently the green process of producing cordycepin and its derivative 3′-deoxyinosine with carbon dioxide can be realized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.