Patent · US Active

Method for adjusting the illumination in a fluorescence microscope, and corresponding fluorescence microscope

US12313830B2 · kind B2 · utility

0Cited by

5References

20Claims

0Family size

Assignee

LEICA MICROSYSTEMS CMS GMBH · DE

Inventors

Christian Schumann · Gießen, DE
Benjamin Deissler · Rockenberg, DE
Kai RITSCHEL · Wetzlar, DE

Key dates

Filing date	Aug 5, 2020
Grant date	May 27, 2025
Priority date	—
Expiry date	Aug 6, 2041

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6441
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and/or cross-excitation of a fluorophore for different illumination spectra of the light sources are/is taken into account.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.