Nanopore voltage methods
US12319960B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 10, 2017 |
| Grant date | Jun 3, 2025 |
| Priority date | — |
| Expiry date | Sep 6, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for sequencing a nucleic acid molecule includes providing a sequencing cell having a nanopore in a membrane that resides over a well, a first electrode at a bottom of the well, a second electrode in a chamber above the membrane, and an electrolyte in the well and the chamber. The first electrode is configured to facilitate non-Faradaic conduction of ionic current and forms a capacitance with ions in the electrolyte. A first voltage signal is applied across the first and second electrodes, thereby creating a force that moves a nucleic acid molecule in the sequence cell through the nanopore. The first voltage signal increases to compensate for changes in the capacitance at the first electrode during application of the first voltage signal. The method further includes determining signal values measured during the first voltage signal, which correspond to one or more nucleotides in the nucleic acid molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.