Methods to spatially profile protease activity in tissue and sections

US12320801B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Sangeeta N. Bhatia · Lexington, US
• Jaideep S. Dudani · Boston, US
• Ester J. Kwon · Cambridge, US
• Andrew David Warren · Cambridge, US

Key dates

• Filing date: Oct 26, 2022
• Grant date: Jun 3, 2025
• Expiry date: Jun 28, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/60
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Aspects of the disclosure relate to methods and compositions useful for in vivo and/or in vitro enzyme profiling. In some embodiments, the disclosure provides methods of in vivo enzymatic processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. In some embodiments, the disclosure provides compositions and in vitro methods for localization of enzymatic activity in a tissue sample.