Characterization of adeno-associated virus using microchip capillary electrophoresis
US12320814B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2021 |
| Grant date | Jun 3, 2025 |
| Priority date | — |
| Expiry date | Nov 14, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/015
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and systems for identifying capsid viral proteins in a sample containing viral vectors are provided, including determining the ratio of the capsid viral proteins of adeno-associated virus. The methods and systems comprise denaturing the capsid viral proteins in the sample, labelling the denatured capsid viral proteins with a lysine-conjugation dye, generating a separation profile of the denatured/labelled capsid viral proteins using microchip capillary electrophoresis, quantifying levels of the capsid viral proteins based on the separation profile, determining a quantification ratio of the capsid viral proteins based on the separation profile, and normalizing the quantification ratio based on lysine contents of the capsid viral proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.