Systems and methods for improved nanopore-based analysis of nucleic acids
US12321837B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 14, 2020 |
| Grant date | Jun 3, 2025 |
| Priority date | — |
| Expiry date | Aug 7, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06N20/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In some embodiments, a computer-implemented method of determining an identity of one or more monomer subunit residues of a polymer analyte is provided, In some embodiments, a raw current signal generated by using a variable voltage to translocate the polymer analyte through a nanopore. In some embodiments, change points are detected in the raw current signal to determine a series of states, In some embodiments, capacitance compensation is performed on the raw current signal for each state to create an ionic current-vs-voltage curve for each state. In some embodiments, the ionic current-vs-voltage curves is converted to conductance-vs-voltage curves. In some embodiments, filtering is performed for the series of states to create a series of filtered states. In some embodiments, the identity of one or more monomer subunit residues of the polymer analyte is determined based on the series of filtered states.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.