Total afucosylated glycoforms of antibodies produced in cell culture
US12325737B2 · kind B2 · utility
0Cited by
127References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 26, 2019 |
| Grant date | Jun 10, 2025 |
| Priority date | — |
| Expiry date | Jun 14, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/732
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods of producing an antibody composition comprising a desired or predetermined or pre-selected level of total afucosylated (TAF) glycoforms. In exemplary embodiments, the method comprises maintaining glycosylation-competent cells in a cell culture medium comprising fucose and/or glucose at a specific concentration as described herein, depending on the level of TAF glycoforms desired. Related compositions comprising glycosylated proteins and TAF glycoforms thereof are also provided herein. Also provided are cell culture media.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.