Degradable microsphere and use thereof
US12325767B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 31, 2019 |
| Grant date | Jun 10, 2025 |
| Priority date | — |
| Expiry date | Sep 29, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2223/418
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.