Enzymatic methods to generate high yields of sequence specific RNA oligonucleotides with extreme precision

US12325873B2 · kind B2 · utility

Assignee

• University of Massachusetts · US

Inventors

• Craig Martin · Amherst, US
• Elvan Cavac · Northampton, US
• Kithmie Harshana Malagodapathiranage · Amherst, US
• Shuo Sui · Amherst, US
• Sarah L. Perry · Granby, US
• Yasaman Gholamalipour · Worcester, US

Key dates

• Filing date: Jan 10, 2023
• Grant date: Jun 10, 2025
• Expiry date: Jun 1, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6865
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.