Thixotropic biocompatible gel for live cell observation in cell computed tomography
US12329382B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 29, 2018 |
| Grant date | Jun 17, 2025 |
| Priority date | — |
| Expiry date | Jun 21, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08J2371/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A thixotropic and biocompatible gel, formed by combining of 4-arm polyethylene glycol with fumed silica (PEG-silica). The thixotropicity of the gel is affected by the ratio of PEG to silica. To study the gel's biocompatibility, the cell suspension in PBS or another medium was added to the PEG-silica gel via mixing to uniformly disperse the cells in the gel and then placed in an incubator before performing fluorescence live-dead assays. Duration of cell viability (cell life time) in gels was measured to be up to several days, depending on gel composition. Due to their optical and fluidic properties the gels are compatible with live cell imaging including 3D computed tomography and offer a means for moving the cells in a highly controllable manner by applying and removing pressure on the gels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.